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Theoretically UV radiation can kill viruses, bacteria, algae, and protozoa. In general, larger organisms, such as protozoa, require a higher dose of UV radiation than smaller organisms, such as bacteria. But there are also differences between various organisms of the same type: some bacteria are more resistant to UV radiation than others.The amount of UV light produced by the bulb is reflected in the wattage of the bulb. Bulbs with a higher wattage produce more UV light. The ability of the germicidal fluorescent lamp to produce UV light decreases with age, and in most cases, the bulb needs replacement every 12 months. UV light is best produced at temperatures of 104-110oF; cooler temperatures will result in less output.